I've implemented internet-based one-time activation on my company's software (C#.net) that requires a license key that refers to a license stored in the server's database. The software hits the server with the key and is given license information that is then encrypted locally using an RSA key generated from some variables (a combination of CPUID and other stuff that won't change often) on the client computer and then stores it in the registry.
It requires some server-side coding, but it has worked really well for us and I was able to use the same system when we expanded to browser-based software.
